https://scholars.lib.ntu.edu.tw/handle/123456789/626231
標題: | Combined fluorophore and phosphor conjugation: a new design concept for simultaneous and spatially localized dual lifetime intracellular sensing of oxygen and pH | 作者: | Solomatina A.I Chelushkin P.S Su S.-H Wu C.-H PI-TAI CHOU Tunik S.P. |
公開日期: | 2022 | 卷: | 58 | 期: | 3 | 起(迄)頁: | 419-422 | 來源出版物: | Chemical Communications | 摘要: | In this communication, we propose a new strategy for double-parametric biosensing and present a dual pH/O2 lifetime sensor based on the covalent conjugation of fluorescein (pH sensor) and an orthometalated iridium complex (O2 sensor) to human serum albumin (HSA). The resulting conjugate demonstrates biocompatibility, low toxicity, and fast cellular uptake, and displays independent lifetime responses towards variations in media acidity and oxygen concentration that makes it suitable for application as an effective pH/O2 probe in luminescence microscopy using the FLIM/PLIM detection mode. The concept applicability has been exemplified using the dual spatially and temporally localized intracellular sensing of pH and O2 concentration in living cells. © The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122724305&doi=10.1039%2fd1cc06132a&partnerID=40&md5=74bb5d86b761f0d11b04c286a406b400 https://scholars.lib.ntu.edu.tw/handle/123456789/626231 |
ISSN: | 13597345 | DOI: | 10.1039/d1cc06132a | SDG/關鍵字: | Iridium compounds; Oxygen; pH sensors; Biosensing; Cellular uptake; Design concept; Human serum albumins; Intracellular sensing; Iridium complex; Localised; Low toxicity; Luminescence microscopy; Oxygen concentrations; Biocompatibility |
顯示於: | 化學系 |
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