|Title:||Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle||Authors:||Su C.-H
|Keywords:||Catharanthus roseus; Imaging mass spectrometry; Petal; Surface-assisted laser desorption/ionization; Titanium dioxide; Vinca alkaloids||Issue Date:||2022||Journal Volume:||2505||Start page/Pages:||45-58||Source:||Methods in molecular biology (Clifton, N.J.)||Abstract:||
The surface-assisted laser desorption/ionization (SALDI) technique uses inorganic materials to aid desorption and ionization of molecules. SALDI is suitable for analyzing small molecules due to the absence of interfering signals in the low m/z range originating from the organic matrix. Imaging mass spectrometry (IMS) is a versatile imaging approach with high spatial resolution for analyzing various molecular species, but its application depends heavily on the ionization method. We have developed a functionalized titanium dioxide (TiO2) nanowire as a solid substrate for SALDI-MS detection of low-molecular-weight molecules. We apply this novel substrate for imprinting fragile specimens such as petals and further SALDI-IMS analysis. The TiO2 nanowire substrate is prepared from a commercial Ti plate by a hydrothermal process and subsequently chemically modified to improve the quality and selectivity of imprinting as well as the sensitivity of SALDI-IMS analysis. Here, the functionalized TiO2 nanowire substrate is applied to visualize the distribution of vinca alkaloids in the petal of Madagascar periwinkle (Catharanthus roseus). © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
|ISSN:||19406029||DOI:||10.1007/978-1-0716-2349-7_3||SDG/Keyword:||Vinca alkaloid; Catharanthus; diagnostic imaging; laser; matrix-assisted laser desorption-ionization mass spectrometry; procedures; Catharanthus; Diagnostic Imaging; Lasers; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Vinca Alkaloids|
|Appears in Collections:||化學系|
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