https://scholars.lib.ntu.edu.tw/handle/123456789/626858
Title: | Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis | Authors: | Wang, Wei-Kung SHEY-YING CHEN Liu, I-Jung YEE-CHUN CHEN Hui-Ling Chen Yang, Chao-Fu PEI-JER CHEN Shiou-Hwei Yeh CHUAN-LIANG KAO LI-MIN HUANG PO-REN HSUEH JANN-TAY WANG WANG-HUEI SHENG CHI-TAI FANG CHIEN-CHING HUNG SZU-MIN HSIEH Su, Chan-Ping WEN-CHU CHIANG Yang, Jyh-Yuan Lin, Jih-Hui Hsieh, Szu-Chia Hu, Hsien-Ping Chiang, Yu-Ping JIN-TOWN WANG PAN-CHYR YANG SHAN-CHWEN CHANG |
Issue Date: | Jul-2004 | Journal Volume: | 10 | Journal Issue: | 7 | Source: | Emerging infectious diseases | Abstract: | The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 x 10(2) to 5.93 x 10(6) copies/mL) and saliva (7.08 x 10(3) to 6.38 x 10(8) copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets. Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/626858 | ISSN: | 1080-6040 | DOI: | 10.3201/eid1007.031113 |
Appears in Collections: | 醫學系 |
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