A Study on the Possibility of Screening the Drug with Antihepadnaviral Effect by Primary Duck Hepatocyte Culture
Other Title
以初代培養鴨肝細胞篩選抗親肝DNA病毒藥物之可行性研究
Journal
Journal of the Chinese Society of Veterinary Science
Journal Volume
22
Journal Issue
3
Pages
192
Date Issued
1996-06-01
Author(s)
Abstract
The aim of this study was evaluate the possibility of primary culture hepatocytes separated from congenitally infected with duck hepatitis B virus (DHBV) ducklings as a tool for screening the drug with anti-hepadnaviral activity. Eleven extracts from four medicinal herbs with strong anti-DHBV endogenous DNA polymerase(DNAP) activity were employed in this study. Changes of the following items were carefully investigated: the hepatocyte morphology; the activities of lactate dehydrogenase and alanine aminotransferase in the culture media; the DHBV-DNA concentration in hepatocytes and in the culture media; and the intermediate products of DHBV-DNA in hepatocytes. Result revealed that this screening system had following excellent characters: not necessary to infect the cells after separation; easy to evaluate the toxicity and the ability to cross the cell membrane of the drugs; and easy to infer the mechanism of anti-DHBV replication of the drug. Therefore, this screening system could be an economic, time-saving, Semi-in vitro and semi-in vivo method to screen a huge number of drugs.
Subjects
鴨B型肝炎病毒
鴨肝細胞培養
抗親肝DNA病毒藥物
DHBV
Duck hepatocyte culture
Anti-hepadnaviral drugs
SDGs
Type
journal article