https://scholars.lib.ntu.edu.tw/handle/123456789/627273
標題: | Characterization of TMAO productivity from carnitine challenge facilitates personalized nutrition and microbiome signatures discovery | 作者: | WEI-KAI WU Panyod, Suraphan Liu, Po-Yu CHIEH-CHANG CHEN HSIEN-LI KAO Chuang, Hsiao-Li YING-HSIEN CHEN Zou, Hsin-Bai Kuo, Han-Chun CHING-HUA KUO Liao, Ben-Yang Chiu, Tina H T Chung, Ching-Hu ANGELA YU-CHEN LIN YI-CHIA LEE Tang, Sen-Lin JIN-TOWN WANG Wu, Yu-Wei CHENG-CHIH HSU LEE-YAN SHEEN Orekhov, Alexander N MING-SHIANG WU |
關鍵字: | Cardiovascular disease; Emergencia timonensis; Gut microbiome; Ihubacter massiliensis; Machine learning; Oral carnitine challenge test; Personalized nutrition; Trimethylamine N-oxide | 公開日期: | 19-十一月-2020 | 出版社: | BMC | 卷: | 8 | 期: | 1 | 起(迄)頁: | 162 | 來源出版物: | Microbiome | 摘要: | The capability of gut microbiota in degrading foods and drugs administered orally can result in diversified efficacies and toxicity interpersonally and cause significant impact on human health. Production of atherogenic trimethylamine N-oxide (TMAO) from carnitine is a gut microbiota-directed pathway and varies widely among individuals. Here, we demonstrated a personalized TMAO formation and carnitine bioavailability from carnitine supplements by differentiating individual TMAO productivities with a recently developed oral carnitine challenge test (OCCT). By exploring gut microbiome in subjects characterized by TMAO producer phenotypes, we identified 39 operational taxonomy units that were highly correlated to TMAO productivity, including Emergencia timonensis, which has been recently discovered to convert γ-butyrobetaine to TMA in vitro. A microbiome-based random forest classifier was therefore constructed to predict the TMAO producer phenotype (AUROC = 0.81) which was then validated with an external cohort (AUROC = 0.80). A novel bacterium called Ihubacter massiliensis was also discovered to be a key microbe for TMA/TMAO production by using an OCCT-based humanized gnotobiotic mice model. Simply combining the presence of E. timonensis and I. massiliensis could account for 43% of high TMAO producers with 97% specificity. Collectively, this human gut microbiota phenotype-directed approach offers potential for developing precision medicine and provides insights into translational research. Video Abstract. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627273 | ISSN: | 2049-2618 | DOI: | 10.1186/s40168-020-00912-y |
顯示於: | 醫學系 |
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