https://scholars.lib.ntu.edu.tw/handle/123456789/627292
標題: | Three-dimensional modelling of the catalytic domain of Streptococcus mutans glucosyltransferase GtfB | 作者: | Tsai, Yau Wei JEAN-SAN CHIA Shiau, Yuh Yuan Chou, Hsiu Chuan Liaw, Yen Chywan KUO-LONG LOU |
關鍵字: | Catalytic domain | Glucosyltransferase | Streptococcus mutans | Sucrase activity | Three-dimensional model | Tim-barrel | 公開日期: | 1-七月-2000 | 卷: | 188 | 期: | 1 | 來源出版物: | FEMS Microbiology Letters | 摘要: | Glucosyltransferases (GtfB/C/D) of Streptococcus mutans, a pathogen for human dental caries, synthesize water-insoluble glucan through the hydrolysis of sucrose. Genetic and biochemical approaches have identified several active sites of these enzymes, but no three-dimensional (3D) structural evidence is yet available to elucidate the subdomain arrangement and molecular mechanism of catalysis. Based on a combined sequence and secondary structure alignment against known crystal structures of segments from closely related proteins, we propose here the 3D model of an N-terminal domain essential for the sucrose binding and splitting in GtfB. A Tim-barrel of (α/β)8 structural characteristics is revealed and the structural correlation for two peptides is described. Copyright (C) 2000 Federation of European Microbiological Societies. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627292 | ISSN: | 03781097 | DOI: | 10.1016/S0378-1097(00)00213-5 |
顯示於: | 臨床牙醫學研究所 |
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