https://scholars.lib.ntu.edu.tw/handle/123456789/627293
標題: | Species identification of mutans streptococci by groESL gene sequence | 作者: | Hung, Wei Chung JUI-CHANG TSAI PO-REN HSUEH JEAN-SAN CHIA LEE-JENE TENG |
關鍵字: | ORAL STREPTOCOCCI | 公開日期: | 1-九月-2005 | 出版社: | MICROBIOLOGY SOC | 卷: | 54 | 期: | 9 | 起(迄)頁: | 857 | 來源出版物: | Journal of Medical Microbiology | 摘要: | The near full-length sequences of the groESL genes were determined and analysed among eight reference strains (serotypes a to h) representing five species of mutans group streptococci. The groES sequences from these reference strains revealed that there are two lengths (285 and 288 bp) in the five species. The intergenic spacer between groES and groEL appears to be a unique marker for species, with a variable size (ranging from 111 to 310 bp) and sequence. Phylogenetic analysis of groES and groEL separated the eight serotypes into two major clusters. Strains of serotypes b, c, e and f were highly related and had groES gene sequences of the same length, 288 bp, while strains of serotypes a, d, g and h were also closely related and their groES gene sequence lengths were 285 bp. The groESL sequences in clinical isolates of three serotypes of S. mutans were analysed for intraspecies polymorphism. The results showed that the groESL sequences could provide information for differentiation among species, but were unable to distinguish serotypes of the same species. Based on the determined sequences, a PCR assay was developed that could differentiate members of the mutans streptococci by amplicon size and provide an alternative way for distinguishing mutans streptococci from other viridans streptococci. © 2005 SGM. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627293 | ISSN: | 00222615 | DOI: | 10.1099/jmm.0.46180-0 |
顯示於: | 臨床牙醫學研究所 |
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