|Title:||Dual Gold-Nanoslit Electrodes for Ultrasensitive Detection of Antigen-Antibody Reactions in Electrochemical Surface Plasmon Resonance||Authors:||Lo, Shu-Cheng
|Keywords:||biointeraction analysis; electrochemistry (EC); gold nanostructures; hyperspectral image; surface plasmon resonance (SPR)||Issue Date:||23-Sep-2022||Publisher:||AMER CHEMICAL SOC||Journal Volume:||7||Journal Issue:||9||Source:||ACS sensors||Abstract:||
We present the use of surface charges in dual gold-nanoslit electrodes to improve the surface plasmon resonance (SPR) detection limit by several orders of magnitude. The SPR is directly generated by gold-nanoslit arrays in the two electrodes. The SPR shifts for both nanoslit arrays are measured simultaneously with a simple hyperspectral setup. When biomolecules are captured by specific antibodies on the dual electrodes, the surface charge is changed during the electrochemical process due to the increase in surface impedance. The push-pull-type electrodes generate opposite surface charges. Using the differences in both spectral shifts, the change in surface charge is detected sensitively. We demonstrate that using a [Fe(CN)6]3-/4- redox process after antigen-antibody interactions, the dual nanoslit electrodes show an enhancement of the detection limit from 1 μg/mL to 10 pg/mL.
|Appears in Collections:||應用力學研究所|
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