https://scholars.lib.ntu.edu.tw/handle/123456789/627847
標題: | Thermosensitive hydrogel carrying extracellular vesicles from adipose-derived stem cells promotes peripheral nerve regeneration after microsurgical repair | 作者: | Chen, Shih-Heng Kao, Huang-Kai Wun, Jing-Ru Chou, Pang-Yun Chen, Zhi-Yu Chen, Shih-Hsien SUNG-TSANG HSIEH Fang, Hsu-Wei Lin, Feng-Huei |
關鍵字: | SCHWANN-CELLS; STRUCTURAL-CHARACTERIZATION; NEUROTROPHIC ACTIVITY; EXOSOMES; INJURY; POLYSACCHARIDE; PROLIFERATION; DELIVERY; BIODISTRIBUTION; ANGIOGENESIS | 公開日期: | 十二月-2022 | 出版社: | AIP Publishing | 卷: | 6 | 期: | 4 | 來源出版物: | APL bioengineering | 摘要: | Peripheral nerve injuries are commonly occurring traumas of the extremities; functional recovery is hindered by slow nerve regeneration (<1 mm/day) following microsurgical repair and subsequent muscle atrophy. Functional recovery after peripheral nerve repair is highly dependent on local Schwann cell activity and axon regeneration speed. Herein, to promote nerve regeneration, paracrine signals of adipose-derived stem cells were applied in the form of extracellular vesicles (EVs) loaded in a thermosensitive hydrogel (PALDE) that could solidify rapidly and sustain high EV concentration around a repaired nerve during surgery. Cell experiments revealed that PALDE hydrogel markedly promotes Schwann-cell migration and proliferation and axon outgrowth. In a rat sciatic nerve repair model, the PALDE hydrogel increased repaired-nerve conduction efficacy; contraction force of leg muscles innervated by the repaired nerve also recovered. Electromicroscopic examination of downstream nerves indicated that fascicle diameter and myeline thickness in the PALDE group (1.91 ± 0.61 and 1.06 ± 0.40 μm, respectively) were significantly higher than those in PALD and control groups. Thus, this EV-loaded thermosensitive hydrogel is a potential cell-free therapeutic modality to improve peripheral-nerve regeneration, offering sustained and focused EV release around the nerve-injury site to overcome rapid clearance and maintain EV bioactivity in vivo. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627847 | ISSN: | 2473-2877 | DOI: | 10.1063/5.0118862 |
顯示於: | 解剖學暨細胞生物學科所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。