https://scholars.lib.ntu.edu.tw/handle/123456789/628125
標題: | Mesenchymal Stem Cells From a Hypoxic Culture Can Improve Rotator Cuff Tear Repair | 作者: | HSIN-SHUI CHEN Yau, Yun-Chain Ko, Pin-Tsou Yen, Betty Lin-Ju Ho, Chun-Te Hung, Shih-Chieh |
關鍵字: | full-thickness rotator cuff tear; hypoxic culture; stem cell | 公開日期: | 2022 | 卷: | 31 | 來源出版物: | Cell transplantation | 摘要: | A rotator cuff tear is an age-related common cause of pain and disability. Studies including our previously published ones have demonstrated that mesenchymal stem cells cultured under hypoxic conditions [hypoxic multipotent stromal cells (MSCs)] facilitate the retention of transplanted cells and promote wound healing. However, there are very few, if any, reports targeting the punctured supraspinatus tendons to create more or equally serous wounds as age-related tears of rotator cuff. It remains to be determined whether transplantation of bone-marrow-derived hypoxic MSCs into the punctured supraspinatus tendon improves tendon repair and, when combined with ultrasound-guided delivery, could be used for future clinical applications. In this study, we used a total of 33 Sprague-Dawley rats in different groups for normal no-punched control, hypoxic MSC treatment, nontreated vehicle control, and MSC preparation, and then evaluated treatment outcomes by biomechanical testing and histological analysis. We found that the ultimate failure load of the hypoxic MSC-treated group was close to that of the normal tendon and significantly greater than that of the nontreated vehicle control group. In vivo tracking of cells labeled with superparamagnetic iron oxide (SPIO) nanoparticles revealed an enhanced retention of transplanted cells at the tear site. Our study demonstrates that hypoxic MSCs improve rotator cuff tear repair in a rat model. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/628125 | ISSN: | 0963-6897 1555-3892 |
DOI: | 10.1177/09636897221089633 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。