https://scholars.lib.ntu.edu.tw/handle/123456789/628772
標題: | Early-onset caloric restriction alleviates ageing-associated steatohepatitis in male mice via restoring mitochondrial homeostasis | 作者: | Chiang, Chun-Hsien Li, Sin-Jin Lin, Yu-Han PEI-YU WANG Hsu, Pu-Sheng SHAU-PING LIN Chiang, Ting-Chia CHING-YI CHEN |
關鍵字: | Ageing; Caloric restriction; Energy deficit; Mitochondrial functions; Steatohepatitis | 公開日期: | 20-二月-2023 | 出版社: | Kluwer Academic | 來源出版物: | Biogerontology | 摘要: | Non-alcoholic fatty liver disease is associated with ageing, and impaired mitochondrial homeostasis is the main cause for hepatic ageing. Caloric restriction (CR) is a promising therapeutic approach for fatty liver. The purpose of the present study was to investigate the possibility of early-onset CR in decelerating the progression of ageing-related steatohepatitis. The putative mechanism associated with mitochondria was further determined. C57BL/6 male mice at 8 weeks of age were randomly assigned to one of three treatments: Young-AL (AL, ad libitum), Aged-AL, or Aged-CR (60% intake of AL). Mice were sacrificed when they were 7 months old (Young) or 20 months old (Aged). Aged-AL mice displayed the greatest body weight, liver weight, and liver relative weight among treatments. Steatosis, lipid peroxidation, inflammation, and fibrosis coexisted in the aged liver. Mega mitochondria with short, randomly organized crista were noticed in the aged liver. The CR ameliorated these unfavourable outcomes. The level of hepatic ATP decreased with ageing, but this was reversed by CR. Ageing caused a decrease in mitochondrial-related protein expressions of respiratory chain complexes (NDUFB8 and SDHB) and fission (DRP1), but an increase in proteins related to mitochondrial biogenesis (TFAM), and fusion (MFN2). CR reversed the expression of these proteins in the aged liver. Both Aged-CR and Young-AL revealed a comparable pattern of protein expression. To summarize, this study demonstrated the potential of early-onset CR in preventing ageing-associated steatohepatitis, and maintaining mitochondrial functions may contribute to CR's protection during hepatic ageing. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/628772 | DOI: | 10.1007/s10522-023-10023-4 |
顯示於: | 腦與心智科學研究所 |
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