https://scholars.lib.ntu.edu.tw/handle/123456789/628784
標題: | Data supporting chitosan facilitates structure formation of the salivary gland by regulating the basement membrane components | 作者: | Hsiao, Ya Chuan TSUNG-LIN YANG |
關鍵字: | Basement membrane | Chitosan | Morphogenesis | Salivary glands | 公開日期: | 1-九月-2015 | 卷: | 4 | 來源出版物: | Data in Brief | 摘要: | To investigate the role of basement membrane (BM) in chitosan-mediated morphogenesis of the salivary glands, the embryonic submandibular gland (SMG) experimental model was used. Chitosan promotes branching at distinct stages in SMG morphogenesis. When enzymes such as type IV collagenase, dispase, and cathepsin B were used to digest the BM components, the morphogenetic effect mediated by chitosan disappeared. Immunofluorescence revealed that the corresponding receptors for BM components, including CD49c, CD49f, CD29, and dystroglycan, were locally enriched at the epithelial-mesenchymal junction around BM areas. The functional roles of laminin α1 and α5 in SMG branching were explored via siRNA knockdown, and suppression was confirmed at both the RNA and protein levels (Yang and Hsiao, Biomaterials, http://dx.doi.org/10.1016/j.biomaterials.2015.06.028, 2015). This data article demonstrates the experimental approaches to investigate the role of basement membrane in the structure formation of the salivary gland engineered by biomaterials. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/628784 | ISSN: | 2352-3409 | DOI: | 10.1016/j.dib.2015.07.006 |
顯示於: | 醫學系 |
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