https://scholars.lib.ntu.edu.tw/handle/123456789/629551
標題: | Crystal structure of a platelet-agglutinating factor isolated from the venom of Taiwan habu (Trimeresurus mucrosquamatus) | 作者: | Huang, Kai-Fa Ko, Tzu-Ping Hung, Chin-Chun Chung-Han Chu Wang, Andrew H-J Chiou, Shyh-Horng |
關鍵字: | Flavocetin-A | Lectin-like protein | Mucrocetin | Platelet glycoprotein Ib | Snake venom | Trimeresurus mucrosquamatus | 公開日期: | 1-三月-2004 | 出版社: | PORTLAND PRESS LTD | 卷: | 378 | 期: | Pt 2 | 起(迄)頁: | 399 | 來源出版物: | The Biochemical journal | 摘要: | Platelet glycoprotein Ib (GPIb)-binding proteins (GPIb-BPs) from snake venoms are usually C-type lectins, which target specific sites of GPIbalpha and elicit distinct effects on platelets. In the present paper, we report a tetrameric platelet-agglutinating factor (molecular mass 121.1 kDa), termed mucrocetin, purified from the venom of Taiwan habu (Trimeresurus mucrosquamatus ). Mucrocetin is a GPIbalpha agonist with a binding site distinct from that of flavocetin-A (a snake venom GPIbalpha antagonist) on GPIbalpha, in spite of the high sequence identity (94.6%) between the two venom lectins. The crystal structure of mucrocetin was solved and refined to 2.8 A (1 A=0.1 nm) resolution, which shows an interesting crystal packing of six-layer cylinders of doughnut-shaped molecules. The four alphabeta heterodimers are arranged in an unusual square-shaped ring stabilized by four interdimer 'head-to-tail' disulphide bridges. Detailed structural comparison between mucrocetin and flavocetin-A suggests that their disparate platelet effects are probably attributable to different charge distributions on the putative concave binding surface. A unique positively charged patch on the binding surface of mucrocetin, formed by Lys102, Lys108, Lys109 and Arg123 in the alpha-subunit coupled with Lys22, Lys102, Lys116 and Arg117 in the beta-subunit, appears to be the primary determinant of its platelet-agglutinating activity. Conceivably, this interesting venom factor may provide a useful tool to study platelet agglutination by binding to the GPIb-IX-V complex. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/629551 | ISSN: | 02646021 | DOI: | 10.1042/BJ20031507 |
顯示於: | 化學系 |
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