https://scholars.lib.ntu.edu.tw/handle/123456789/630077
標題: | The effect of sertoli cells on xenotransplantation and allotransplantation of ventral mesencephalic tissue in a rat model of parkinson’s disease | 作者: | Jhao, Yun Ting Chiu, Chuang Hsin Chen, Chien Fu F. Chou, Ta Kai Lin, Yi Wen YU-TEN JU SHINN-CHIH WU Yan, Ruoh Fang Shiue, Chyng Yann Chueh, Sheau Huei Halldin, Christer Cheng, Cheng Yi Ma, Kuo Hsing |
關鍵字: | Parkinson’s disease | Positron emission tomography | Sertoli cell | Transplantation | Ventral mesencephalic tissue | 公開日期: | 1-十一月-2019 | 出版社: | MDPI | 卷: | 8 | 期: | 11 | 來源出版物: | Cells | 摘要: | Intra-striatal transplantation of fetal ventral mesencephalic (VM) tissue has a therapeutic effect on patients with Parkinson’s disease (PD). Sertoli cells (SCs) possess immune-modulatory properties that benefit transplantation. We hypothesized that co-graft of SCs with VM tissue can attenuate rejection. Hemi-parkinsonian rats were generated by injecting 6-hydroxydopamine into the right medial forebrain bundle of Sprague Dawley (SD) rats. The rats were then intrastriatally transplanted with VM tissue from rats or pigs (rVM or pVM), with/without a co-graft of SCs (rVM+SCs or pVM+SCs). Recovery of dopaminergic function and survival of the grafts were evaluated using the apomorphine-induced rotation test and small animal-positron emission tomography (PET) coupled with [18F] DOPA or [18F] FE-PE2I, respectively. Immunohistochemistry (IHC) examination was used to determine the survival of the grafted dopaminergic neurons in the striatum and to investigate immune-modulatory effects of SCs. The results showed that the rVM+SCs and pVM+SCs groups had significantly improved drug-induced rotational behavior compared with the VM alone groups. PET revealed a significant increase in specific uptake ratios (SURs) of [18F] DOPA and [18F] FE-PE2I in the grafted striatum of the rVM+SCs and pVM+SCs groups as compared to that of the rVM and pVM groups. SC and VM tissue co-graft led to better dopaminergic (DA) cell survival. The co-grafted groups exhibited lower populations of T-cells and activated microglia compared to the groups without SCs. Our results suggest that co-graft of SCs benefit both xeno-and allo-transplantation of VM tissue in a PD rat model. Use of SCs enhanced the survival of the grafted dopaminergic neurons and improved functional recovery. The enhancement may in part be attributable to the immune-modulatory properties of SCs. In addition, [18F]DOPA and [18F]FE-PE2I coupled with PET may provide a feasible method for in vivo evaluation of the functional integrity of the grafted DA cell in parkinsonian rats. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088206915&doi=10.3390%2fcells8111420&partnerID=40&md5=c74c82805fb542d628a6c9281896ec20 https://scholars.lib.ntu.edu.tw/handle/123456789/630077 |
ISSN: | 2073-4409 | DOI: | 10.3390/cells8111420 | SDG/關鍵字: | allotransplantation; animal cell; animal model; Article; behavioral test parameters; cell maturation; confocal microscopy; controlled study; corpus callosum; corpus striatum; dopaminergic activity; human; human tissue; immunohistochemistry; immunomodulation; medial forebrain bundle; mesencephalic tissue; nonhuman; Parkinson disease; positron emission tomography; rat; Sertoli cell; xenotransplantation; animal; disease model; immunology; male; mesencephalon; metabolism; Parkinson disease; pathology; Sertoli cell; T lymphocyte; transplantation; xenograft |
顯示於: | 動物科學技術學系 |
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