https://scholars.lib.ntu.edu.tw/handle/123456789/630245
標題: | In vitro culture and characterization of duck primordial germ cells | 作者: | YI-CHEN CHEN SHAU-PING LIN Chang, Yi Ying Chang, Wei Peng Wei, Liang Yuan Liu, Hsiu Chou Huang, Jeng Fang Pain, Bertrand SHINN-CHIH WU |
關鍵字: | Mule duck (C. moschata × A. platyrhynchos) | Muscovy duck (Cairina moschata) | Pekin duck (Anas platyrhynchos) | Primordial germ cell (PGC) | 公開日期: | 1-一月-2019 | 出版社: | OXFORD UNIV PRESS | 卷: | 98 | 期: | 4 | 起(迄)頁: | 1820 | 來源出版物: | Poultry Science | 摘要: | This study aimed to isolate, culture, and characterize duck primordial germ cells (PGCs) and to compare these cells with chicken PGCs. We first cultured Muscovy duck (Cairina moschata) circulating PGCs and gonadal PGCs (gPGCs) in the modified serum-containing medium used to amplify chicken PGCs. gPGCs were found to proliferate better in serum-free chemically defined medium than in serum-containing medium. Thereafter, gPGCs were similarly isolated from 2 other duck breeds, the Pekin duck (Anas platyrhynchos) and the hybrid mule duck (C. moschata × A. platyrhynchos), and amplified for a limited period of time in the chemically defined culture condition, but sufficiently to be characterized and transplanted. Cultured gPGCs of all 3 duck breeds were characterized by Periodic acid-Schiff staining, immunocytochemical staining, and expression analysis of germline-specific and pluripotency genes. Cultured duck gPGCs colonized the gonads after being genetically labeled and injected into recipient embryos. Taken together, these results demonstrate that duck PGCs retain their germline characteristics after being isolated, expanded in vitro, and genetically modified. Further studies are required to establish the optimal conditions for long-term culture of duck PGCs, which may involve supplementing the culture medium with other growth factors or compounds. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062872985&doi=10.3382%2fps%2fpey515&partnerID=40&md5=077a03a39ba7e059e268d90cd990e325 https://scholars.lib.ntu.edu.tw/handle/123456789/630245 |
ISSN: | 00325791 | DOI: | 10.3382/ps/pey515 |
顯示於: | 動物科學技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。