Airy light sheet microscope for in-vivo imaging of C. elegans
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
11925
ISBN
9781510647190
Date Issued
2021-01-01
Author(s)
Abstract
The feature of rapid divergence of conventional Gaussian beam restricts the field of view in light sheet microscopy. Comparison with Gaussian beam, the Airy beams provides many advantages in light sheet microscopy such as larger field of view (FOV), longer penetration path, and self-reconstruction. C. elegans is a model organism for developmental biological studies. Here, we design Airy light sheet microscope to perform in-vivo imaging of C. elegans. A spatial light modulator is used to generate 1D Airy beam for illumination purpose. Imaging performance is experimentally evaluated for our system. Present results may find important applications for studying internal structures in-vivo imaging of C. elegans.
Subjects
Airy beam | C. Elegans | Light sheet fluorescence microscopy
Type
conference paper