https://scholars.lib.ntu.edu.tw/handle/123456789/630551
標題: | Biological properties of human periodontal ligament cell spheroids cultivated on chitosan and polyvinyl alcohol membranes | 作者: | Hsieh, Hao Ying JANE CHUNG-CHEN YAO Hsu, Li Fang Tsai, Li Hui JIIANG-HUEI JENG Young, Tai-Horng YI-JANE CHEN |
關鍵字: | Cell migration | Chitosan | Hypoxia-inducible factor 1 | Paxillin | Polyvinyl alcohol | 公開日期: | 1-十一月-2022 | 出版社: | ELSEVIER TAIWAN | 卷: | 121 | 期: | 11 | 起(迄)頁: | 2191 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background/purpose: Multicellular spheroid cultures have attracted increasing attention in the field of periodontal regeneration. However, very few studies have reported the periodontal ligament (PDL) cell spheroid formation via biomaterials-induced processes. This study investigated the biological characteristics of human PDL cell spheroids formed on two hydrophilic polymer-based biomaterials, namely chitosan and polyvinyl alcohol. Methods: The expressions of periostin, paxillin, hypoxia-inducible factor 1-α (HIF-1α), and vascular endothelial growth factor (VEGF) were analyzed. Cell migration ability was assessed using a scratch assay. Furthermore, PDL cell spheroids were cultured in 3D-printed polylactic acid scaffolds to evaluate mineralizing capability. Results: Western blot analysis revealed increased expressions of periostin, HIF-1α, and VEGF in the 3D spheroids. After the spheroids were reseeded, the cells gradually migrated outward from the spheroids and time-dependent distribution of paxillin was observed. The cells migrating outward from the 3D spheroids demonstrated greater migration ability than that of 2D monolayer cells. Compared to the dissociated cells from a monolayer culture, the cell spheroids formed on the chitosan membrane exhibited elevated alkaline phosphatase activity and an increase in mineralized matrix deposition. Conclusion: The biomaterial-induced formation of PDL cell spheroids suggests a novel strategy for cell delivery in research and clinical applications of periodontal regeneration. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/630551 | ISSN: | 09296646 | DOI: | 10.1016/j.jfma.2022.03.014 |
顯示於: | 牙醫學系 |
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