https://scholars.lib.ntu.edu.tw/handle/123456789/630621
標題: | Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient | 作者: | Zhou, Daming Duyvesteyn, Helen M.E. Chen, Cheng Pin Huang, Chung Guei Chen, Ting Hua Shih, Shin Ru Lin, Yi Chun Cheng, Chien Yu Cheng, Shu Hsing Huang, Yhu Chering Lin, Tzou Yien Ma, Che Huo, Jiandong Carrique, Loic Malinauskas, Tomas Ruza, Reinis R. Shah, Pranav N.M. Tan, Tiong Kit Rijal, Pramila Donat, Robert F. Godwin, Kerry Buttigieg, Karen R. Tree, Julia A. Radecke, Julika Paterson, Neil G. Supasa, Piyada Mongkolsapaya, Juthathip Screaton, Gavin R. Carroll, Miles W. Gilbert-Jaramillo, Javier Knight, Michael L. James, William Owens, Raymond J. Naismith, James H. Townsend, Alain R. Fry, Elizabeth E. Zhao, Yuguang Ren, Jingshan Stuart, David I. Kuan-Ying A. Huang |
關鍵字: | NANOLITRE CRYSTALLIZATION EXPERIMENTS; CRYO-EM STRUCTURE; SARS CORONAVIRUS; POTENT NEUTRALIZATION; PROTEIN; SPIKE | 公開日期: | 1-十月-2020 | 出版社: | NATURE PORTFOLIO | 卷: | 27 | 期: | 10 | 起(迄)頁: | 950 | 來源出版物: | Nature Structural and Molecular Biology | 摘要: | The COVID-19 pandemic has had an unprecedented health and economic impact and there are currently no approved therapies. We have isolated an antibody, EY6A, from an individual convalescing from COVID-19 and have shown that it neutralizes SARS-CoV-2 and cross-reacts with SARS-CoV-1. EY6A Fab binds the receptor binding domain (RBD) of the viral spike glycoprotein tightly (KD of 2 nM), and a 2.6-Å-resolution crystal structure of an RBD–EY6A Fab complex identifies the highly conserved epitope, away from the ACE2 receptor binding site. Residues within this footprint are key to stabilizing the pre-fusion spike. Cryo-EM analyses of the pre-fusion spike incubated with EY6A Fab reveal a complex of the intact spike trimer with three Fabs bound and two further multimeric forms comprising the destabilized spike attached to Fab. EY6A binds what is probably a major neutralizing epitope, making it a candidate therapeutic for COVID-19. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/630621 | ISSN: | 15459993 | DOI: | 10.1038/s41594-020-0480-y |
顯示於: | 免疫學研究所 |
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s41594-020-0480-y.pdf | Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient | 8.95 MB | Adobe PDF | 檢視/開啟 |
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