https://scholars.lib.ntu.edu.tw/handle/123456789/630980
標題: | Spatial and temporal dynamics of ATP synthase from mitochondria toward the cell surface | 作者: | Chang, Yi-Wen Tony Yang, T Chen, Min-Chun Liaw, Y-Geh Yin, Chieh-Fan Lin-Yan, Xiu-Qi Huang, Ting-Yu Hou, Jen-Tzu Hung, Yi-Hsuan Hsu, Chia-Lang Huang, Hsuan-Cheng HSUEH-FEN JUAN |
關鍵字: | AXONAL-TRANSPORT; PLASMA-MEMBRANE; ANGIOGENESIS INHIBITOR; MALATE-DEHYDROGENASE; THERAPEUTIC TARGET; CITRATE SYNTHASE; FISSION; FUSION; DRP1; EXPRESSION | 公開日期: | 18-四月-2023 | 出版社: | NATURE PORTFOLIO | 卷: | 6 | 期: | 1 | 起(迄)頁: | Article number 427 | 來源出版物: | Communications biology | 摘要: | Ectopic ATP synthase complex (eATP synthase), located on cancer cell surface, has been reported to possess catalytic activity that facilitates the generation of ATP in the extracellular environment to establish a suitable microenvironment and to be a potential target for cancer therapy. However, the mechanism of intracellular ATP synthase complex transport remains unclear. Using a combination of spatial proteomics, interaction proteomics, and transcriptomics analyses, we find ATP synthase complex is first assembled in the mitochondria and subsequently delivered to the cell surface along the microtubule via the interplay of dynamin-related protein 1 (DRP1) and kinesin family member 5B (KIF5B). We further demonstrate that the mitochondrial membrane fuses to the plasma membrane in turn to anchor ATP syntheses on the cell surface using super-resolution imaging and real-time fusion assay in live cells. Our results provide a blueprint of eATP synthase trafficking and contribute to the understanding of the dynamics of tumor progression. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/630980 | ISSN: | 2399-3642 | DOI: | 10.1038/s42003-023-04785-3 |
顯示於: | 生命科學系 |
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