https://scholars.lib.ntu.edu.tw/handle/123456789/631029
標題: | Self-Healing of Recombinant Spider Silk Gel and Coating | 作者: | Wu, Shin-Da Chuang, Wei-Tsung Ho, Jo-Chen HSUAN-CHEN WU SHAN-HUI HSU |
關鍵字: | in situ SAXS; recombinant spider silk; self-healing; β-sheet nanocrystal | 公開日期: | 12-四月-2023 | 出版社: | MDPI | 卷: | 15 | 期: | 8 | 起(迄)頁: | Article number 1855 | 來源出版物: | Polymers | 摘要: | Self-healing properties, originating from the natural healing process, are highly desirable for the fitness-enhancing functionality of biomimetic materials. Herein, we fabricated the biomimetic recombinant spider silk by genetic engineering, in which Escherichia coli (E. coli) was employed as a heterologous expression host. The self-assembled recombinant spider silk hydrogel was obtained through the dialysis process (purity > 85%). The recombinant spider silk hydrogel with a storage modulus of ~250 Pa demonstrated autonomous self-healing and high strain-sensitive properties (critical strain ~50%) at 25 °C. The in situ small-angle X-ray scattering (in situ SAXS) analyses revealed that the self-healing mechanism was associated with the stick-slip behavior of the β-sheet nanocrystals (each of ~2-4 nm) based on the slope variation (i.e., ~-0.4 at 100%/200% strains, and ~-0.9 at 1% strain) of SAXS curves in the high q-range. The self-healing phenomenon may occur through the rupture and reformation of the reversible hydrogen bonding within the β-sheet nanocrystals. Furthermore, the recombinant spider silk as a dry coating material demonstrated self-healing under humidity as well as cell affinity. The electrical conductivity of the dry silk coating was ~0.4 mS/m. Neural stem cells (NSCs) proliferated on the coated surface and showed a 2.3-fold number expansion after 3 days of culture. The biomimetic self-healing recombinant spider silk gel and thinly coated surface may have good potential in biomedical applications. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/631029 | ISSN: | 2073-4360 | DOI: | 10.3390/polym15081855 |
顯示於: | 生化科技學系 |
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