Using BLAST in molecular species identification of fungi 1: Practical guidelines
Journal
NOVA HEDWIGIA
Journal Volume
116
Journal Issue
1-2
Pages
67
Date Issued
2023
Author(s)
Abstract
DNA sequence-based species identification and phylogeny have become indispensable in biological sciences. The DNA sequence databases, however, are flooded with more data than can be curated, leaving increasing responsibility to the users in order to minimize errors due to wrong information. Since many publications deal with the principles of DNA barcoding, but do not practically explain the verification of BLAST results, we here present some simple guidelines from a taxonomical view and experience as users for other users. We suggest eight recommendations for using BLAST to ensure that the most appropriate names according to the state of art are attributed to the sequences and provide examples.
Subjects
Environmental sequences; ITS; MegaBLAST; molecular species recognition; type; vouchers
SDGs
Publisher
GEBRUDER BORNTRAEGER
Type
journal article