https://scholars.lib.ntu.edu.tw/handle/123456789/631037
標題: | Canine transmissible venereal tumour established in immunodeficient mice reprograms the gene expression profiles associated with a favourable tumour microenvironment to enable cancer malignancy | 作者: | Ke, Chiao Hsu Tomiyasu, Hirotaka Lin, Yu Ling WEI-HSIANG HUANG Huang, Hsiao Hsuan Chiang, Hsin Chien CHEN-SI LIN |
關鍵字: | Canine tumour model; cDNA microarray; Immunoediting; Infectious cancer; Tumour biomarkers | 公開日期: | 1-十二月-2022 | 出版社: | BMC | 卷: | 18 | 期: | 1 | 來源出版物: | BMC Veterinary Research | 摘要: | Background: Canine transmissible venereal tumours (CTVTs) can cross the major histocompatibility complex barrier to spread among dogs. In addition to the transmissibility within canids, CTVTs are also known as a suitable model for investigating the tumour–host immunity interaction because dogs live with humans and experience the same environmental risk factors for tumourigenesis. Moreover, outbred dogs are more appropriate than inbred mice models for simulating the diversity of human cancer development. This study built a new model of CTVTs, known as MCTVTs, to further probe the shaping effects of immune stress on tumour development. For xenotransplantation, CTVTs were first injected and developed in immunodeficient mice (NOD.CB17-Prkdcscid/NcrCrl), defined as XCTVTs. The XCTVTs harvested from NOD/SCID mice were then inoculated and grown in beagles and named mouse xenotransplantation of CTVTs (MCTVTs). Results: After the inoculation of CTVTs and MCTVTs into immune-competent beagle dogs separately, MCTVTs grew faster and metastasized more frequently than CTVTs did. Gene expression profiles in CTVTs and MCTVTs were analysed by cDNA microarray to reveal that MCTVTs expressed many tumour-promoting genes involved in chronic inflammation, chemotaxis, extracellular space modification, NF-kappa B pathways, and focal adhesion. Furthermore, several well-known tumour-associated biomarkers which could predict tumour progression were overexpressed in MCTVTs. Conclusions: This study demonstrated that defective host immunity can result in gene instability and enable transcriptome reprogramming within tumour cells. Fast tumour growth in beagle dogs and overexpression of tumour-associated biomarkers were found in a CTVT strain previously established in immunodeficient mice. In addition, dysregulated interaction of chronic inflammation, chemotaxis, and extracellular space modification were revealed to imply the possibly exacerbating mechanisms in the microenvironments of these tumours. In summary, this study offers a potential method to facilitate tumour progression and provide a niche for discovering tumour-associated biomarkers in cancer research. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122177607&doi=10.1186%2fs12917-021-03093-4&partnerID=40&md5=d8a91d31b1e2bbc4adc7d32cf826bf4b https://scholars.lib.ntu.edu.tw/handle/123456789/631037 |
ISSN: | 1746-6148 | DOI: | 10.1186/s12917-021-03093-4 | SDG/關鍵字: | animal cell; animal experiment; animal model; animal tissue; Article; beagle; bioinformatics; cancer model; cancer research; Canidae; canine transmissible venereal tumor; carcinogenesis; chemotaxis; chronic inflammation; clinical article; cytology; differential gene expression; DNA microarray; dog breed; focal adhesion; gene cluster; gene expression profiling; gene ontology; gene overexpression; genomic instability; histology; histopathology; human; major histocompatibility complex; male; malignant neoplasm; mitosis; mouse; NF kB signaling; nonhuman; protein expression; protein protein interaction; real time polymerase chain reaction; SCID mouse; signal transduction; transcriptome sequencing; tumor growth; tumor immunity; tumor microenvironment; xenotransplantation; animal; animal venereal tumor; dog; dog disease; genetics; inflammation; nonobese diabetic mouse; veterinary medicine |
顯示於: | 獸醫學系 |
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