https://scholars.lib.ntu.edu.tw/handle/123456789/631041
標題: | Xenograft cancer vaccines prepared from immunodeficient mice increase tumor antigen diversity and host T cell efficiency against colorectal cancers | 作者: | Ke, Chiao Hsu Wang, Yu Shan Chiang, Hsin Chien Wu, Hsin-Yi Liu, Wang Jing Huang, Cheng Chung Huang, Yi Chun CHEN-SI LIN |
關鍵字: | Autologous cancer vaccines (ACVs); Immunogenicity; Personalized medicine; Th-1 immunity; Tumor-associated antigens (TAAs) | 公開日期: | 1-二月-2022 | 出版社: | ELSEVIER IRELAND LTD | 卷: | 526 | 起(迄)頁: | 66 | 來源出版物: | Cancer Letters | 摘要: | Autologous cancer vaccines (ACVs) are a desirable approach for personalized medicine, but the efficiency of ACVs remains unsatisfactory due to their low immunogenicity. This study developed a platform that can enhance the immunogenicity of ACVs by transplanting the tumors into immunodeficient mice. The CT26 cell line was inoculated into severe combined immunodeficient mice (SCID) for vaccine preparation where escalates tumor development, subsequently diversifying the tumor antigenic topology. CT26/SCID cancer vaccines significantly inhibited tumor growth, increased the amount of tumor infiltrating lymphocytes, and triggered Th-1 predominant immune responses. Tumor antigenic profiles of CT26/SCID cells were further analyzed by liquid chromatography–tandem mass spectrometry. Compared to CT26 parental cells, a total of 428 differentially expressed proteins (DEPs) were detected. These DEPs revealed that CT26/SCID cells overexpressed several novel therapeutic targets, including KNG1, apoA-I and, β2-GPI, which can trigger cytotoxic T cells towards Th-1 predominant immune responses and directly suppress proliferation in tumors. CT26/SCID cancer vaccines can be easily manufactured, while traits of triggering stronger antigen-specific Th-1 immune activity against tumors, are retained. Results of this study provide an effective proof-of-concept of an ACV for personalized cancer immunotherapy. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119516233&doi=10.1016%2fj.canlet.2021.11.012&partnerID=40&md5=7d86c1f9537ddf940e17184781a43a3b https://scholars.lib.ntu.edu.tw/handle/123456789/631041 |
ISSN: | 03043835 | DOI: | 10.1016/j.canlet.2021.11.012 |
顯示於: | 獸醫學系 |
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