https://scholars.lib.ntu.edu.tw/handle/123456789/631186
標題: | Highly Mimetic Ex Vivo Lung-Cancer Spheroid-Based Physiological Model for Clinical Precision Therapeutics | 作者: | Shie, Ming-You Fang, Hsin-Yuan Kan, Kai-Wen Ho, Chia-Che Tu, Chih-Yen Lee, Pei-Chih PO-REN HSUEH Chen, Chia-Hung Lee, Alvin Kai-Xing Tien, Ni Chen, Jian-Xun Shen, Yu-Cheng Chang, Jan-Gowth Shen, Yu-Fang Lin, Ting-Ju Wang, Ben Hung, Mien-Chie Cho, Der-Yang Chen, Yi-Wen |
關鍵字: | decellularized extracellular matrix; lung spheroid model; precision medicine; tumor microenvironment | 公開日期: | 21-四月-2023 | 出版社: | WILEY | 來源出版物: | Advanced science (Weinheim, Baden-Wurttemberg, Germany) | 摘要: | Lung cancer remains a major health problem despite the considerable research into prevention and treatment methods. Through a deeper understanding of tumors, patient-specific ex vivo spheroid models with high specificity can be used to accurately investigate the cause, metastasis, and treatment strategies for lung cancer. Biofabricate lung tumors are presented, consisting of patient-derived tumor spheroids, endothelial cells, and lung decellularized extracellular matrix, which maintain a radial oxygen gradient, as well as biophysicochemical behaviors of the native tumors for precision medicine. It is also demonstrated that the developed lung-cancer spheroid model reproduces patient responses to chemotherapeutics and targeted therapy in a co-clinical trial, with 85% accuracy, 86.7% sensitivity, and 80% specificity. RNA sequencing analysis validates that the gene expression in the spheroids replicates that in the patient's primary tumor. This model can be used as an ex vivo predictive model for personalized cancer therapy and to improve the quality of clinical care. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/631186 | ISSN: | 2198-3844 | DOI: | 10.1002/advs.202206603 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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