https://scholars.lib.ntu.edu.tw/handle/123456789/631295
標題: | Plasmonic nanostructure-enhanced Raman scattering for detection of SARS-CoV-2 nucleocapsid protein and spike protein variants | 作者: | Yeh, Yi Jui Le, Trong Nghia Hsiao, Wesley Wei Wen KUO-LUN TUNG Ostrikov, Kostya (Ken) Chiang, Wei Hung |
關鍵字: | Pathogen detection | Point-of-care test | Raman spectroscopy | Rapid diagnosis | SARS-CoV-2 | SERS | 公開日期: | 25-一月-2023 | 出版社: | ELSEVIER | 卷: | 1239 | 來源出版物: | Analytica Chimica Acta | 摘要: | Epidemiological control and public health monitoring during the outbreaks of infectious viral diseases rely on the ability to detect viral pathogens. Here we demonstrate a rapid, sensitive, and selective nanotechnology-enhanced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection based on the surface-enhanced Raman scattering (SERS) responses from the plasma-engineered, variant-specific antibody-functionalized silver microplasma-engineered nanoassemblies (AgMEN) interacting with the SARS-CoV-2 spike (S) and nucleocapsid (N) proteins. The three-dimensional (3D) porous AgMEN with plasmonic-active nanostructures provide a high sensitivity to virus detection via the remarkable SERS signal collection. Moreover, the variant-specific antibody-functionalization on the SERS-active AgMEN enabled the high selectivity of the SARS-CoV-2 S variants, including wild-type, Alpha, Delta, and Omicron, under the simulated human saliva conditions. The exceptional ultrahigh sensitivity of our SERS biosensor was demonstrated via SARS-CoV-2 S and N proteins at the detection limit of 1 fg mL−1 and 0.1 pg mL−1, respectively. Our work demonstrates a versatile SERS-based detection platform can be applied for the ultrasensitive detection of virus variants, infectious diseases, and cancer biomarkers. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/631295 | ISSN: | 00032670 | DOI: | 10.1016/j.aca.2022.340651 |
顯示於: | 化學工程學系 |
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