https://scholars.lib.ntu.edu.tw/handle/123456789/631734
標題: | Localization and quantification of endoplasmic reticulum Ca(2+)-ATPase isoform transcripts | 作者: | KWAN-DUN WU Lee, W S Wey, J Bungard, D Lytton, J |
關鍵字: | in situ hybridization | quantitative Northern blotting | sarcoplasmic or endoplasmic reticulum calcium-adenosinetriphosphatase isoforms | 公開日期: | 九月-1995 | 卷: | 269 | 期: | 3 Pt 1 | 來源出版物: | The American journal of physiology | 摘要: | The Ca(2+)-adenosinetriphosphatase pump of the sarcoplasmic or endoplasmic reticulum (SERCA) plays a critical role in Ca2+ signaling and homeostasis in all cells and is encoded by a family of homologous and alternatively spliced genes. To understand more clearly the role the different isoforms play in cell physiology, we have undertaken a quantitative and qualitative assessment of the tissue distribution of transcripts encoding each SERCA isoform. SERCA1 expression is restricted to fast-twitch striated muscles, SERCA2a to cardiac and slow-twitch striated muscles, whereas SERCA2b is ubiquitously expressed. SERCA3 is expressed most abundantly in large and small intestine, thymus, and cerebellum and at lower levels in spleen, lymph node, and lung. In situ hybridization analyses revealed SERCA3 transcripts in cells of the intestinal crypt, the thymic cortex, and Purkinje cells in cerebellum. In addition, SERCA3 was expressed abundantly in isolated rat spleen lymphocytes, in various murine lymphoid cell lines, and in primary cultured microvascular endothelial cells. This analysis demonstrates that SERCA3 is expressed selectively in cells in which Ca2+ signaling plays a critical and sensitive role in regulating physiological processes. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/631734 | ISSN: | 0002-9513 | DOI: | 10.1152/ajpcell.1995.269.3.C775 |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。