https://scholars.lib.ntu.edu.tw/handle/123456789/632027
標題: | PEDOT:PSS-Based Bioelectrodes for Multifunctional Drug Release and Electric Cell-Substrate Impedance Sensing | 作者: | Hsiao, Yu Sheng Quiñones, Edgar Daniel Yen, Shih Chieh JIASHING YU Fang, Ji Tseng Chen, Peilin Juang, Ruey Shin |
關鍵字: | conducting polymer (CP) | drug release | drug screening | electric cell-substrate impedance sensing (ECIS) | poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) | 公開日期: | 1-一月-2023 | 出版社: | AMER CHEMICAL SOC | 卷: | 15 | 期: | 18 | 起(迄)頁: | 21953 | 來源出版物: | ACS Applied Materials and Interfaces | 摘要: | Electric cell-substrate impedance sensing (ECIS) is an innovative approach for the label-free and real-time detection of cell morphology, growth, and apoptosis, thereby playing an essential role as both a viable alternative and valuable complement to conventional biochemical/pharmaceutical analysis in the field of diagnostics. Constant improvements are naturally sought to further improve the effective range and reliability of this technology. In this study, we developed poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) conducting polymer (CP)-based bioelectrodes integrated into homemade ECIS cell-culture chamber slides for the simultaneous drug release and real-time biosensing of cancer cell viability under drug treatment. The CP comprised tailored PEDOT:PSS, poly(ethylene oxide) (PEO), and (3-glycidyloxypropyl)trimethoxysilane (GOPS) capable of encapsulating antitumor chemotherapeutic agents such as doxorubicin (DOX), docetaxel (DTX), and a DOX/DTX combination. This device can reliably monitor impedance signal changes correlated with cell viability on chips generated by cell adhesion onto a predetermined CP-based working electrode while simultaneously exhibiting excellent properties for both drug encapsulation and on-demand release from another CP-based counter electrode under electrical stimulation (ES) operation. Cyclic voltammetry curves and surface profile data of different CP-based coatings (without or with drugs) were used to analyze the changes in charge capacity and thickness, respectively, thereby further revealing the correlation between their drug-releasing performance under ES operation (determined using ultraviolet-visible (UV-vis) spectroscopy). Finally, antitumor drug screening tests (DOX, DTX, and DOX/DTX combination) were performed on MCF-7 and HeLa cells using our developed CP-based ECIS chip system to monitor the impedance signal changes and their related cell viability results. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/632027 | ISSN: | 19448244 | DOI: | 10.1021/acsami.3c02769 |
顯示於: | 化學工程學系 |
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