On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio
Journal
PLoS ONE
Journal Volume
9
Journal Issue
6
Date Issued
2014
Author(s)
Abstract
Upon injury, the direct damage and the subsequent secondary injury in the brain often result in chronic neurological disorders. Due to multifactorial nature of secondary injury and subsequent complex cellular responses, much of the underlying mechanisms are unclear. This study used an adult zebrafish cerebellum injury model to investigate the phenotypes and the secondary injury responses for recovery mechanisms of injured brain. Using the time course microarray analysis, a candidate protein-protein interaction (PPI) network was refined as cerebellar wound healing PPI network by dynamic modeling and big data mining. Pathway enrichment and ontological analysis were incorporated into the refined network to highlight the main molecular scheme of cerebellar wound healing. Several significant pathways, including chemokine, Phosphatidylinositide 3-kinases, and axon guidance signaling pathway and their cross-talks through PI3K, PAK2, and PLXNA3 were identified to coordinate for neurogenesis and angiogenesis, which are essential for the restoration of the injured brain. Our finding provides an insight into the molecular restoration mechanisms after traumatic brain injury, and open up new opportunity to devise the treatment for traumatic brain injury in human. © 2014 Wu et al.
Other Subjects
chemokine; p21 activated kinase 2; phosphatidylinositol 3 kinase; plexin; plexin A3; unclassified drug; phosphatidylinositol 3 kinase; zebrafish protein; angiogenesis; animal experiment; animal model; article; cerebellum cortex; controlled study; microarray analysis; molecular interaction; nerve fiber; nervous system development; nonhuman; ontology; phenotype; protein analysis; protein protein interaction; signal transduction; traumatic brain injury; wound healing; zebra fish; animal; biological model; brain injury; cerebellum; disease model; human; metabolism; pathophysiology; procedures; protein microarray; proteomics; Animals; Brain Injuries; Cerebellum; Disease Models, Animal; Humans; Models, Neurological; Neurogenesis; Phosphatidylinositol 3-Kinase; Protein Array Analysis; Protein Interaction Maps; Proteomics; Signal Transduction; Wound Healing; Zebrafish; Zebrafish Proteins
Type
journal article