https://scholars.lib.ntu.edu.tw/handle/123456789/632679
標題: | Identification of silk components in the bombycoid moth Andraca theae (Endromidae) reveals three fibroin subunits resembling those of Bombycidae and Sphingidae | 作者: | Kmet, Peter Kucerova, Lucie Sehadova, Hana Chia-Hsiang Wu, Bulah YUEH-LUNG WU Zurovec, Michal |
關鍵字: | Adhesives; Bombyx mori; Mucin; P25; Saturniidae; Sericin; Seroin; Zonadhesin-like | 公開日期: | 六月-2023 | 卷: | 147 | 來源出版物: | Journal of insect physiology | 摘要: | The silk produced by Lepidoptera caterpillars is a mixture of proteins secreted by the transformed labial glands, the silk glands (SG). The silk fiber consists of insoluble filamentous proteins that form a silk core and are produced in the posterior part of the SG and soluble coat proteins consisting of sericins and various other polypeptides secreted in the middle part of the SG. We constructed a silk gland specific transcriptome of Andraca theae and created a protein database required for peptide mass fingerprinting. We identified major silk components by proteomic analysis of cocoon silk and by searching for homologies with known silk protein sequences from other species. We identified 30 proteins including a heavy chain fibroin, a light chain fibroin and fibrohexamerin (P25) that form the silk core, as well as members of several structural families that form the silk coating. To uncover the evolutionary relationships among silk proteins, we included orthologs of silk genes from several recent genome projects and performed phylogenetic analyses. Our results confirm the recent molecular classification that the family Endromidae appears to be slightly more distant from the family Bombycidae. Our study provides important information on the evolution of silk proteins in the Bombycoidea, which is needed for proper annotation of the proteins and future functional studies. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/632679 | ISSN: | 00221910 | DOI: | 10.1016/j.jinsphys.2023.104523 |
顯示於: | 昆蟲學系 |
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