https://scholars.lib.ntu.edu.tw/handle/123456789/632992
標題: | Material extrusion-based 3D printing for the fabrication of bacteria into functional biomaterials: The case study of ammonia removal application | 作者: | Li, Yan Peng, Shuqiang Li, Kunrong Qin, Dan Weng, Zixiang Li, Jiangwei Zheng, Longhui Wu, Lixin CHANG-PING YU |
關鍵字: | Ammonia removal | Bio-ink | Dual crosslinking | Heterotrophic bacteria | Material extrusion-based 3D printing | 公開日期: | 1-十二月-2022 | 出版社: | ELSEVIER | 卷: | 60 | 來源出版物: | Additive Manufacturing | 摘要: | 3D printing provides a new way of microbial immobilization technology and creates innovative designed bioactive structures containing microorganisms for remediation of environmental pollution. However, the bio-ink design remains a critical challenge due to the difficulty in creating a durable and bio-friendly material. Here, a novel dual-crosslinking PEGDA-Alginate-PVA-Nanoclay (PAPN) bio-ink containing one heterotrophic bacterium is reported for 3D printing functional biomaterial with the capabilities of ammonia removal. The results showed that PAPN 3D printed bio-scaffolds could effectively remove 96.2 ± 1.3% ammonia within 12 h, and the removal rate increased within repeated use owing to the growth of bacteria inside the bio-scaffolds. Preservation of the bio-scaffolds under room temperature without a culture medium for 168 h still maintained the microbial activity for ammonia removal. It is demonstrated that visible-light-based 3D printing procedures could maintain high cell viability for the majority of bacteria, and the porous structures of 3D bio-scaffolds could allow the permeability of nutrients for the growth of bacteria. This work demonstrates the potential of the dual crosslinked PAPN 3D bio-scaffold for the production and application of immobilized functional bacteria in wastewater treatment. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/632992 | ISSN: | 22148604 | DOI: | 10.1016/j.addma.2022.103268 |
顯示於: | 環境工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。