https://scholars.lib.ntu.edu.tw/handle/123456789/633270
標題: | Probing Folate-Responsive and Stage-Sensitive Metabolomics and Transcriptional Co-Expression Network Markers to Predict Prognosis of Non-Small Cell Lung Cancer Patients | 作者: | Lin, Yu-Shun Chen, Yen-Chu Chen, Tzu-En Cheng, Mei-Ling Lynn, Ke-Shiuan Shah, Pramod JIN-SHING CHEN Huang, Rwei-Fen S |
關鍵字: | WGCNA; non–small-cell lung cancers; target metabolomics; transcriptional profile; tumour folate | 公開日期: | 20-十二月-2022 | 卷: | 15 | 期: | 1 | 來源出版物: | Nutrients | 摘要: | Tumour metabolomics and transcriptomics co-expression network as related to biological folate alteration and cancer malignancy remains unexplored in human non-small cell lung cancers (NSCLC). To probe the diagnostic biomarkers, tumour and pair lung tissue samples (n = 56) from 97 NSCLC patients were profiled for ultra-performance liquid chromatography tandem mass spectrometry (UPLC/MS/MS)-analysed metabolomics, targeted transcriptionomics, and clinical folate traits. Weighted Gene Co-expression Network Analysis (WGCNA) was performed. Tumour lactate was identified as the top VIP marker to predict advance NSCLC (AUC = 0.765, Sig = 0.017, CI 0.58-0.95). Low folate (LF)-tumours vs. adjacent lungs displayed higher glycolytic index of lactate and glutamine-associated amino acids in enriched biological pathways of amino sugar and glutathione metabolism specific to advance NSCLCs. WGCNA classified the green module for hub serine-navigated glutamine metabolites inversely associated with tumour and RBC folate, which module metabolites co-expressed with a predominant up-regulation of LF-responsive metabolic genes in glucose transport (GLUT1), de no serine synthesis (PHGDH, PSPH, and PSAT1), folate cycle (SHMT1/2 and PCFR), and down-regulation in glutaminolysis (SLC1A5, SLC7A5, GLS, and GLUD1). The LF-responsive WGCNA markers predicted poor survival rates in lung cancer patients, which could aid in optimizing folate intervention for better prognosis of NSCLCs susceptible to folate malnutrition. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633270 | ISSN: | 2072-6643 | DOI: | 10.3390/nu15010003 |
顯示於: | 醫學系 |
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