https://scholars.lib.ntu.edu.tw/handle/123456789/633394
標題: | miR-194 Up-Regulates Cholesterol 7 Alpha-Hydroxylase Expression via β-Catenin Signaling and Aggravates Cholestatic Liver Diseases | 作者: | Chen, Po-Chun Hsu, Chien-Peng Wang, Sheng-Ya Wu, Tsai-Yen Lin, Yu-Jyun YOU-TZUNG CHEN SHU-HAO HSU |
公開日期: | 六月-2023 | 出版社: | Elsevier Inc. | 卷: | 193 | 期: | 6 | 起(迄)頁: | 755 - 768 | 來源出版物: | The American Journal of Pathology | 摘要: | miR-194 is abundantly expressed in hepatocytes, and its depletion increases hepatic resistance to acetaminophen-induced acute injuries. In this study, the biological role of miR-194 in cholestatic liver injury was investigated by using miR-194/miR-192 cluster liver-specific knockout (LKO) mice, in which no liver injuries or metabolic disorders were predisposed. Bile duct ligation (BDL) and 1-naphthyl isothiocyanate (ANIT) were applied to LKO and matched control wild-type (WT) mice to induce hepatic cholestasis. Periportal liver damage, mortality rate, and liver injury biomarkers in LKO mice were significantly less than in WT mice after BDL and ANIT injection. Intrahepatic bile acid level was significantly lower in the LKO liver within 48 hours of BDL- and ANIT-induced cholestasis compared with WT. Western blot analysis showed that β-catenin (CTNNB1) signaling and genes involved in cellular proliferation were activated in BDL- and ANIT-treated mice. The expression levels of cholesterol 7 alpha-hydroxylase (CYP7A1), pivotal in bile synthesis, and its upstream regulator hepatocyte nuclear factor 4α were reduced in primary LKO hepatocytes and liver tissues compared with WT. The knockdown of miR-194 using miRNA inhibitors reduced CYP7A1 expression in WT hepatocytes. In contrast, the knockdown of CTNNB1 and overexpression of miR-194, but not miR-192, in LKO hepatocytes and AML12 cells increased CYP7A1 expression. In conclusion, the results suggest that the loss of miR-194 ameliorates cholestatic liver injury and may suppress CYP7A1 expression via activation of CTNNB1 signaling. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633394 | ISSN: | 00029440 | DOI: | 10.1016/j.ajpath.2023.02.007 |
顯示於: | 解剖學暨細胞生物學科所 |
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