https://scholars.lib.ntu.edu.tw/handle/123456789/633546
標題: | Transparent tissue in solid state for solvent-free and antifade 3D imaging | 作者: | Hsiao, Fu-Ting Chien, Hung-Jen Chou, Ya-Hsien Peng, Shih-Jung Chung, Mei-Hsin Huang, Tzu-Hui Lo, Li-Wen Shen, Chia-Ning Chang, Hsiu-Pi CHIH-YUAN LEE CHIEN-CHIA CHEN YUNG-MING JENG YU-WEN TIEN Tang, Shiue-Cheng |
公開日期: | 9-六月-2023 | 卷: | 14 | 期: | 1 | 來源出版物: | Nature communications | 摘要: | Optical clearing with high-refractive-index (high-n) reagents is essential for 3D tissue imaging. However, the current liquid-based clearing condition and dye environment suffer from solvent evaporation and photobleaching, causing difficulties in maintaining the tissue optical and fluorescent features. Here, using the Gladstone-Dale equation [(n-1)/density=constant] as a design concept, we develop a solid (solvent-free) high-n acrylamide-based copolymer to embed mouse and human tissues for clearing and imaging. In the solid state, the fluorescent dye-labeled tissue matrices are filled and packed with the high-n copolymer, minimizing scattering in in-depth imaging and dye fading. This transparent, liquid-free condition provides a friendly tissue and cellular environment to facilitate high/super-resolution 3D imaging, preservation, transfer, and sharing among laboratories to investigate the morphologies of interest in experimental and clinical conditions. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633546 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-023-39082-4 |
顯示於: | 醫學系 |
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