https://scholars.lib.ntu.edu.tw/handle/123456789/633650
標題: | Development of Multiplex RT-PCR Assay for the Simultaneous Detection of Four Systemic Diseases Infecting Citrus | 作者: | Yao, Shun Min Wu, Meng Ling TING-HSUAN HUNG |
關鍵字: | citrus exocortis | citrus Huanglongbing | citrus tatter leaf | citrus tristeza | multiplex RT-PCR | 公開日期: | 1-六月-2023 | 卷: | 13 | 期: | 6 | 來源出版物: | Agriculture (Switzerland) | 摘要: | The citrus systemic diseases, including citrus Huanglongbing (caused by Candidatus Liberibacter asiaticus (CLas)), citrus tristeza (caused by citrus tristeza virus (CTV)), citrus tatter leaf (caused by citrus tatter leaf virus (CTLV)), and citrus exocortis (caused by citrus exocortis viroid (CEVd)), are threats to citrus production in Taiwan. Reliable diagnostic methods are important for the management of these systemic diseases. In this study, we developed a multiplex reverse transcription–polymerase chain reaction (RT-PCR) assay to detect four pathogens simultaneously. Herein, the specific amplicons from each pathogen (295 bp for CLas, 468 bp for CTV, 120 bp for CTLV, and 196 bp for CEVd) were successfully produced using the optimized multiplex RT-PCR described here. The sensitivity evaluation showed that low titers of pathogens could be detected using this multiplex RT-PCR. Compared with the published simplex assays, the detection of field samples using the multiplex RT-PCR developed in this study showed a better performance. The detections using multiplex RT-PCR revealed that these four citrus systemic pathogens were commonly found in fields, and 30.0% of field samples were mix-infected. To our knowledge, this is the first study of a survey of the four important citrus systemic diseases in Taiwan, and it provides insights for improving disease management. Therefore, the multiplex RT-PCR assay provides a useful method for routine disease surveying and the production of pathogen-free citrus plants. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633650 | ISSN: | 2077-0472 | DOI: | 10.3390/agriculture13061227 |
顯示於: | 植物病理與微生物學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。