https://scholars.lib.ntu.edu.tw/handle/123456789/633839
標題: | Effect of high-frequency low-intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway | 作者: | Chen, Wei-Ting Lin, Guan-Bo Kuo, Yu-Yi Hsieh, Chih-Hsiung Lu, Chueh-Hsuan Sun, Yi-Kun CHIH-YU CHAO |
公開日期: | 2021 | 卷: | 16 | 期: | 4 | 來源出版物: | PloS one | 摘要: | As the most common type of neurodegenerative diseases (NDDs), Alzheimer's disease (AD) is thought to be caused mainly by the excessive aggregation of β-amyloid protein (Aβ). However, a growing number of studies have found that reactive oxygen species (ROS) play a key role in the onset and progression of AD. The present study aimed to probe the neuroprotective effect of high-frequency low-intensity pulsed electric field (H-LIPEF) for SH-SY5Y cells against hydrogen peroxide (H2O2) and Aβ-induced cytotoxicity. By looking in a systematic way into the frequency- and amplitude-dependent neuroprotective effect of pulsed electric field (PEF), the study finds that H-LIPEF at 200 Hz produces the optimal protective effect for SH-SY5Y cells. The underlying mechanisms were confirmed to be due to the activation of extracellular signal-regulated kinase (ERK) pathway and the downstream prosurvival and antioxidant proteins. Because the electric field can be modified to focus on specific area in a non-contact manner, the study suggests that H-LIPEF holds great potential for treating NDDs, whose effect can be further augmented with the administering of drugs or natural compounds at the same time. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633839 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0250491 |
顯示於: | 物理學系 |
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