https://scholars.lib.ntu.edu.tw/handle/123456789/634068
標題: | Overexpression of adiponectin receptor 1 inhibits brown and beige adipose tissue activity in mice | 作者: | Chen, Yu-Jen Lin, Chiao Wei Peng, Yu Ju Huang, Chao Wei Chien, Yi Shan Huang, Tzu Hsuan Liao, Pei Xin WEN-YUAN YANG Wang, Mei Hui Mersmann, Harry J. SHINN-CHIH WU TAI-YUAN CHUANG YUAN-YU LIN WEN-HUNG KUO SHIH-TORNG DING |
關鍵字: | Adiponectin | Adiponectin receptor 1 | Beige adipose tissue | Brown adipose tissue | Cold-induced thermogenesis | PET/CT scintigraphy | Uncoupling protein 1 (UCP1) | 公開日期: | 2-一月-2021 | 卷: | 22 | 期: | 2 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Adult humans and mice possess significant classical brown adipose tissues (BAT) and, upon cold-induction, acquire brown-like adipocytes in certain depots of white adipose tissues (WAT), known as beige adipose tissues or WAT browning/beiging. Activating thermogenic classical BAT or WAT beiging to generate heat limits diet-induced obesity or type-2 diabetes in mice. Adiponectin is a beneficial adipokine resisting diabetes, and causing “healthy obese” by increasing WAT expansion to limit lipotoxicity in other metabolic tissues during high-fat feeding. However, the role of its receptors, especially adiponectin receptor 1 (AdipoR1), on cold-induced thermogenesis in vivo in BAT and in WAT beiging is still elusive. Here, we established a cold-induction procedure in transgenic mice overexpressing AdipoR1 and applied a live 3-D [18F] fluorodeoxyglucose-PET/CT (18F-FDG PET/CT) scanning to measure BAT activity by determining glucose uptake in cold-acclimated transgenic mice. Results showed that cold-acclimated mice over-expressing AdipoR1 had diminished coldinduced glucose uptake, enlarged adipocyte size in BAT and in browned WAT, and reduced surface BAT/body temperature in vivo. Furthermore, decreased gene expression, related to thermogenic Ucp1, BAT-specific markers, BAT-enriched mitochondrial markers, lipolysis and fatty acid oxidation, and increased expression of whitening genes in BAT or in browned subcutaneous inguinal WAT of AdipoR1 mice are congruent with results of PET/CT scanning and surface body temperature in vivo. Moreover, differentiated brown-like beige adipocytes isolated from pre-adipocytes in subcutaneous WAT of transgenic AdipoR1 mice also had similar effects of lowered expression of thermogenic Ucp1, BAT selective markers, and BAT mitochondrial markers. Therefore, this study combines in vitro and in vivo results with live 3-D scanning and reveals one of the many facets of the adiponectin receptors in regulating energy homeostasis, especially in the involvement of cold-induced thermogenesis. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100158749&doi=10.3390%2fijms22020906&partnerID=40&md5=4dbb1066d213f198829a828ac9ca923e https://scholars.lib.ntu.edu.tw/handle/123456789/634068 |
ISSN: | 16616596 | DOI: | 10.3390/ijms22020906 |
顯示於: | 動物科學技術學系 |
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