https://scholars.lib.ntu.edu.tw/handle/123456789/634491
標題: | Natural fucoidans inhibit coronaviruses by targeting viral spike protein and host cell furin | 作者: | Yang, Cheng-Wei Hsu, Hsing-Yu Lee, Yue-Zhi Jan, Jia-Tsrong SUI-YUAN CHANG Lin, Yi-Ling Yang, Ruey-Bing TAI-LING CHAO Liang, Jian-Jong Lin, Shu-Jing Liao, Chun-Che Chang, Chih-Shin Sytwu, Huey-Kang Hung, Ming-Shiu Chen, Chiung-Tong Lee, Shiow-Ju |
關鍵字: | COVID-19; Coronavirus; Fucoidan; Furin; OC43; SARS-CoV-2 | 公開日期: | 21-七月-2023 | 卷: | 215 | 來源出版物: | Biochemical pharmacology | 摘要: | Fucoidans are a class of long chain sulfated polysaccharides and have multiple biological functions. Herein, four natural fucoidans extracted from Fucus vesiculosus, F. serratus, Laminaria japonica and Undaria pinnatifida, were tested for their HCoV-OC43 inhibition and found to demonstrate EC50 values ranging from 0.15 to 0.61 µg/mL. That from U. pinnatifida exhibited the most potent anti-HCoV-OC43 activity with an EC50 value of 0.15 ± 0.02 µg/mL, a potency largely independent of its sulfate content. Comparison of the gene expression profiles of fucoidan-treated and untreated cells infected with HCoV-OC43 revealed that fucoidan treatment effectively diminished HCoV-OC43 gene expressions associated with induced chemokines, cytokines and viral activities. Further studies using a highly fucoidan-resistant HCoV-OC43 determined that fucoidan inhibited HCoV-OC43 infection via interfering with viral entry and led to the identification of the specific site on the N-terminal region of spike protein, that located adjacent to the host cell receptor binding domain, targeted by the virus. Furthermore, in a SARS-CoV-2 pseudovirus neutralization assay, fucoidan also blocked SARS-CoV-2 entry. In vitro and in vivo, fucoidan decreased SARS-CoV-2 viral loads and inhibited viral infection in Calu-3 or Vero E6 cells and SARS-CoV-2 infected hamsters, respectively. Fucoidan was also found to inhibit furin activity, and reported furin inhibitors were found to inhibit viral infection by wild type HCoV-OC43 or SARS-CoV-2. Accordingly, we conclude that fucoidans inhibit coronaviral infection by targeting viral spike protein and host cell furin to interfere with viral entry. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/634491 | ISSN: | 00062952 | DOI: | 10.1016/j.bcp.2023.115688 |
顯示於: | 醫學檢驗暨生物技術學系 |
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