https://scholars.lib.ntu.edu.tw/handle/123456789/634645
標題: | Direct in situ and real-time visualization of salt-dependent thiolated-DNA immobilization and biosensing on gold in nanofluidic channels | 作者: | Yang, Deng Kai Yu, Liang Kun Kuo, Chia Tzu Weng, Jui Hong Leïchlé, Thierry Swami, Nathan S. LIN-CHI CHEN Wei, Pei Kuen Chou, Chia Fu |
關鍵字: | Biosensing | Depletion zone/effect | in situ visualization | Nanofluidics | Thiolated-DNA–gold conjugation | 公開日期: | 1-十一月-2023 | 卷: | 394 | 來源出版物: | Sensors and Actuators B: Chemical | 摘要: | Thiol-gold (Au) conjugation is perhaps the most routinely applied surface chemistry approach to immobilize receptors within Au-based molecular sensing platforms. However, no previous studies have investigated in situ immobilization in a nanofluidic systems. In this work, flowthrough receptor immobilization is demonstrated, using a nanoslit induced depletion zone to enable direct real-time visualization for characterizing the effect of sodium chloride (NaCl) concentrations during immobilization of thiolated single-stranded DNA on Au. The results show that the amount of fixed-DNA on Au, as well as the non-specific adsorption of DNA on fused silica surface, strongly depend on NaCl levels. Furthermore, the non-specific interaction at high NaCl levels reduces the DNA available for immobilization on Au, but enriches the DNA molecules in the channel under wash by deionized water. By adding Tween-20 to decrease the non-specific adsorption and increasing the driving pressure to increase the DNA flux under high-salt level, the immobilization time can be further decreased to 10 min or less, which is at least an order of magnitude faster than recently proposed bioconjugation methods for thiolated-DNA–Au conjugation. Moreover, this work demonstrates the capability of the platform for DNA- and aptamer-based sensing assays, thereby opening up new strategies for developing biosensors using nanofluidic platforms. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85166586961&doi=10.1016%2fj.snb.2023.134303&partnerID=40&md5=3d2be6f5dbfcbbe7bd85393869fa8b2d https://scholars.lib.ntu.edu.tw/handle/123456789/634645 |
ISSN: | 09254005 | DOI: | 10.1016/j.snb.2023.134303 |
顯示於: | 生物機電工程學系 |
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