https://scholars.lib.ntu.edu.tw/handle/123456789/634760
標題: | Isolation, full sequence analysis, and in situ hybridization of pigeon paramyxovirus-1 genotype VI.2.1.1.2.2 from oriental turtle doves (Streptopelia orientalis) | 作者: | Wang, Han Yang Wu, Meng Chi HUI-WEN CHEN Lai, Yun Chiang WEI-HSIANG HUANG HUI-WEN CHANG Jeng, Chain Ren Cheng, Chiu Hung Wang, Pao Jung Lai, Yen Hsueh YEN-CHEN CHANG |
關鍵字: | avian paramyxovirus-1 | pigeon paramyxovirus type 1 | Streptopelia orientalis | 公開日期: | 1-十月-2023 | 卷: | 102 | 期: | 10 | 來源出版物: | Poultry Science | 摘要: | Pigeon paramyxovirus-1 (PPMV-1), a genetic variant of avian paramyxovirus-1 (APMV-1), has been identified in Columbiformes and is the primary cause of diseases in captive and free-ranging pigeons. However, it has also been reported that PPMV-1 can infect chickens naturally and experimentally, thus posing a potential threat to the poultry industry. This study investigated a lethal outbreak of paramyxovirus infection that occurred among 16 oriental turtle doves (Streptopelia orientalis) in a walk-in aviary at a zoo from March to April 2021. Necropsies were performed, and histopathological findings revealed mild to moderate lymphoplasmacytic infiltration in several organs, such as the pancreas, liver, kidneys, and lungs. Reverse transcription polymerase chain reaction (RT-PCR) using formalin-fixed paraffin-embedded tissue blocks, virus isolation from fresh tissue, and in situ hybridization against the fusion (F) protein confirmed the diagnosis for PPMV-1 infection. The isolated strain NTU/C239/21 was fully sequenced by next-generation sequencing, and the results of phylogenetic analyses revealed that the F protein of NTU/C239/21 shared 98.8% nucleotide sequence identity with Pigeon/Taiwan/AHRI121/2017, which was isolated from a feral pigeon in Taiwan. The present study is the first to identify PPMV-1 infection in Streptopelia orientalis and suggests that Streptopelia orientalis may also play an important role in spreading the infection, similar to pigeons in APMV-1 spreading. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85167455465&doi=10.1016%2fj.psj.2023.102974&partnerID=40&md5=c839db37c3750d1a671ef4d90013376d https://scholars.lib.ntu.edu.tw/handle/123456789/634760 |
ISSN: | 00325791 | DOI: | 10.1016/j.psj.2023.102974 |
顯示於: | 分子暨比較病理生物學研究所 |
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