https://scholars.lib.ntu.edu.tw/handle/123456789/634926
標題: | Enhanced exopolysaccharide production of Cordyceps militaris via mycelial cell immobilization on plastic composite support in repeated-batch fermentation | 作者: | Lin, Shin-Ping Sung, Ting-Hsuan Angkawijaya, Artik Elisa Go, Alchris Woo Hsieh, Chang-Wei Hsu, Hsien-Yi Santoso, Shella Permatasari KUAN-CHEN CHENG |
關鍵字: | Cordycepin; Fermentation; Mycelium cell; Repeated batch; Submerged culture | 公開日期: | 10-八月-2023 | 卷: | 250 | 來源出版物: | International journal of biological macromolecules | 摘要: | Repeated-batch fermentation with fungal mycelia immobilized in plastic composite support (PCS) eliminates the lag phase during fermentation and improves metabolite productivity. The strategy is implemented herein, and a novel modified PCS is developed to enhance exopolysaccharide (EPS) production from the medicinal fungus Cordyceps militaris. A modified PCS (SYE + PCS) was made by compositing polypropylene (PP) with a nutrient mixture containing soybean hull, peptone, yeast extract, and minerals (SYE+). The use of SYE + PCS has consistent cell productivity throughout the multiple fermentation cycles, which resulted in a more higher cell productivity after second batch compared to unmodified PCS. The cell grown on SYE + PCS also generates a higher yield of EPS (3.36, 6.93, and 5.72 g/L in the first, second, and third fermentation cycles, respectively) up to three-fold higher than the cell immobilized on unmodified PCS. It is also worth noting that the EPS from mycelium grown on SYE + PCS contains up to 2.3-fold higher cordycepin than those on unmodified PCS. The presence of nutrients in SYE + PCS also affects the hydrophobicity and surface roughness of the PC, improving mycelial cell adhesion. This study also provides a preliminary antioxidant activity assessment of EPS from immobilized C. militaris grown with SYE + PCS. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/634926 | ISSN: | 01418130 | DOI: | 10.1016/j.ijbiomac.2023.126267 |
顯示於: | 生物科技研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。