https://scholars.lib.ntu.edu.tw/handle/123456789/635388
標題: | Amperometric L-glutamate sensor using a novel L-glutamate oxidase from Streptomyces platensis NTU 3304 | 作者: | Chen, Chien-Yuan Yuan-Chi, Su |
關鍵字: | Amperometry | Enzyme electrodes | Glutamate | 公開日期: | 1-一月-1991 | 卷: | 243 | 期: | C | 起(迄)頁: | 9 | 來源出版物: | Analytica Chimica Acta | 摘要: | Streptomyces platensis NTU 3304, isolated from soil samples, produces extracellular L-glutamate oxidase in liquid culture. Strains of this species have never been reported to be able to produce this enzyme. The purified enzyme was immobilized onto a cellulose triacetate membrane which was held at an oxygen electrode. The sensor was specific to L-glutamate in accordance with the properties of the novel L-glutamate oxidase. The time required for each assay in batch operation was less than 3 min. A linear relationship is observed between the decrease in dissolved oxygen and the concentration of L-glutamate between 20 and 140 mg l-1 (ca. 0.12 and 0.84 mM). The sensor retained 95% of its original activity after 400 assays over a period of 3 weeks. The sensor was applied to determine the concentration of L-glutamate in broth samples during L-glutamic acid fermentation. Good correlations were achieved between results obtained with the sensor and by enzymatic analysis using glutamate dehydrogenase. © 1991. |
URI: | https://www.scopus.com/record/display.uri?eid=2-s2.0-0026021960&doi=10.1016%2fS0003-2670%2800%2982534-9&origin=inward&txGid=64c20bac10671b43caef06bdb26531d6 https://scholars.lib.ntu.edu.tw/handle/123456789/635388 |
ISSN: | 00032670 | DOI: | 10.1016/S0003-2670(00)82534-9 |
顯示於: | 醫學系 |
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