Determination of o-phthalaldehyde for dose verification of the clinical disinfectant by fluorescent sequential injection analysis
Journal
Analytical Sciences
Date Issued
2023-01-01
Author(s)
Abstract
A new automated, generic analytical approach for determining the clinical disinfectant o-phthalaldehyde (OPA) is reported in this study. The proposed sequential injection analysis (SIA) is based on the online reaction of the OPA with glycine/N-acetylcysteine (NAC) in a neutral medium (pH = 7.0) to form a highly fluorescent isoindole derivative. All critical flow and reaction variables were investigated, while validation was carried out in the linearity detection range (0.0075–0.02%). As a result, excellent linearity (R 2 > 0.99) and precision (1.5–2.4% for repeatability and 0.7–2.2% for reproducibility) were achieved for the reference OPA solutions. Furthermore, reasonable concentration verification of OPA disinfection (0.2–0.6%) in healthcare institutes can be achieved using the developed fluorescent SIA due to its good sensitivity (0.111 V/%) and precision (1.0–2.3% for intermediate precision) around the minimum effective concentration (MEC) of 0.3% for Cidex-OPA disinfectant. Graphical abstract: [Figure not available: see fulltext.]
Subjects
Disinfectant | Fluorimetry | o-Phthalaldehyde (OPA) | Sequential injection analysis (SIA)
SDGs
Type
journal article