Microfluidic shear stress-induced differentiation of human stem cells into endothelial cells
Journal
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
ISBN
9780979806421
Date Issued
2009-01-01
Author(s)
Tsai, Po Chun
Chung, Yu Wei
Wang, Yu Hung
Chiang, Han Yun
Yuan, Chen Ching
Hwang, Shiaw Min
Huang, Long Sun
Abstract
This paper shows the effect of microfluidic shear stress on the culture of endothelial cells and the endothelial differentiation of multi-potent amniotic fluid stem cells after shear stress stimulation. Amniotic fluid stem cells were cultured in veinsized microfluidic channels and were induced with shear stress. The entire process has been documented under the microscope system (Fig. 1). The results showed the expression of CD31 and vWF for AFSC after 3 hours of 12 dyne/cm2 shear stress after three hours and a significant alignment for endothelial cells after 24 hours of 12 dyne/cm2 shear stress. © 2009 CBMS.
Subjects
Shear stress | Stem cell differentiation | Vessel remodelling
Type
conference paper