In Vitro Microfluidics-based Blood-brain Barrier Model

Organs-on-chips in vitro model is a micro engineering system that containing the microchannel structure and could replicate the tissue living condition in vivo. Its benefit is to construct the user-friendly platform for the further biological experiment. We demonstrate a low-cost 2D organ-on-chip model which mimic the blood brain barrier (BBB) tissue for further clinical experiment, such as the drug delivery and cytotoxicity threat. MEMS-fabricated wafer was used for the microchannel fabrication. The completely transparent material offered the advanced visibility for the researchers. The continuous medium supplement could mimic the hydrodynamic shear stress (SS) as the physical stimulus to the cell. We described the positive correlation between the shear stress and cell growing orientation.