https://scholars.lib.ntu.edu.tw/handle/123456789/637205
標題: | NME3 binds to phosphatidic acid and mediates PLD6-induced mitochondrial tethering | 作者: | Su, You-An Chiu, Hsin-Yi Chang, Yu-Chen Sung, Chieh-Ju Chen, Chih-Wei Tei, Reika Huang, Xuang-Rong Hsu, Shao-Chun Lin, Shan-Shan Wang, Hsien-Chu Lin, Yu-Chun Hsu, Jui-Cheng Bauer, Hermann Feng, Yuxi Baskin, Jeremy M ZEE-FEN CHANG YA-WEN LIU |
公開日期: | 2-十月-2023 | 出版社: | Rockefeller University Press | 卷: | 222 | 期: | 10 | 來源出版物: | The Journal of Cell Biology | 摘要: | Mitochondria are dynamic organelles regulated by fission and fusion processes. The fusion of membranes requires elaborative coordination of proteins and lipids and is particularly crucial for the function and quality control of mitochondria. Phosphatidic acid (PA) on the mitochondrial outer membrane generated by PLD6 facilitates the fusion of mitochondria. However, how PA promotes mitochondrial fusion remains unclear. Here, we show that a mitochondrial outer membrane protein, NME3, is required for PLD6-induced mitochondrial tethering or clustering. NME3 is enriched at the contact interface of two closely positioned mitochondria depending on PLD6, and NME3 binds directly to PA-exposed lipid packing defects via its N-terminal amphipathic helix. The PA binding function and hexamerization confer NME3 mitochondrial tethering activity. Importantly, nutrient starvation enhances the enrichment efficiency of NME3 at the mitochondrial contact interface, and the tethering ability of NME3 contributes to fusion efficiency. Together, our findings demonstrate NME3 as a tethering protein promoting selective fusion between PLD6-remodeled mitochondria for quality control. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/637205 | ISSN: | 00219525 | DOI: | 10.1083/jcb.202301091 |
顯示於: | 分子醫學研究所 |
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