https://scholars.lib.ntu.edu.tw/handle/123456789/637208
標題: | Rege-1 promotes C. elegans survival by modulating IIS and TOR pathways | 作者: | Tsai, Yi-Ting Chang, Chen-Hsi HSIN-YUE TSAI |
公開日期: | 八月-2023 | 出版社: | Public Library of Science | 卷: | 19 | 期: | 8 | 來源出版物: | PLoS Genetics | 摘要: | Metabolic pathways are known to sense the environmental stimuli and result in physiological adjustments. The responding processes need to be tightly controlled. Here, we show that upon encountering P. aeruginosa, C. elegans upregulate the transcription factor ets-4, but this upregulation is attenuated by the ribonuclease, rege-1. As such, mutants with defective REGE-1 ribonuclease activity undergo ets-4-dependent early death upon challenge with P. aeruginosa. Furthermore, mRNA-seq analysis revealed associated global changes in two key metabolic pathways, the IIS (insulin/IGF signaling) and TOR (target of rapamycin) kinase signaling pathways. In particular, failure to degrade ets-4 mRNA in activity-defective rege-1 mutants resulted in upregulation of class II longevity genes, which are suppressed during longevity, and activation of TORC1 kinase signaling pathway. Genetic inhibition of either pathway way was sufficient to abolish the poor survival phenotype in rege-1 worms. Further analysis of ETS-4 ChIP data from ENCODE and characterization of one upregulated class II gene, ins-7, support that the Class II genes are activated by ETS-4. Interestingly, deleting an upregulated Class II gene, acox-1.5, a peroxisome β-oxidation enzyme, largely rescues the fat lost phenotype and survival difference between rege-1 mutants and wild-types. Thus, rege-1 appears to be crucial for animal survival due to its tight regulation of physiological responses to environmental stimuli. This function is reminiscent of its mammalian ortholog, Regnase-1, which modulates the intestinal mTORC1 signaling pathway. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/637208 | ISSN: | 15537390 | DOI: | 10.1371/journal.pgen.1010869 |
顯示於: | 分子醫學研究所 |
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