https://scholars.lib.ntu.edu.tw/handle/123456789/637262
標題: | Canine diffuse large b-cell lymphoma downregulates the activity of CD8 + T-cells through tumor-derived extracellular vesicles | 作者: | Weng, Hsin Pei Ke, Chiao Hsu Tung, Chun Wei Tani, Akiyoshi CHIA-CHI WANG WEN-YUAN YANG Wang, Yu Shan Han, Winston Liao, Chi Hsun Tomiyasu, Hirotaka CHEN-SI LIN |
關鍵字: | CD8 + T-cell | CTLA-4 blockade | Diffuse large B-cell lymphoma | Extracellular vesicles | Immune suppression | 公開日期: | 1-十二月-2023 | 卷: | 23 | 期: | 1 | 來源出版物: | Cancer Cell International | 摘要: | Background: Tumor-derived extracellular vesicles (EVs) have been proposed as the essential mediator between host immunity and cancer development. These EVs conduct cellular communication to facilitate tumor growth, enable invasion and metastasis, and shape the favorable tumor microenvironment. Lymphoma is one of the most common hematological malignancies in humans and dogs. Effective T-cell responses are required for the control of these malignancies. However, the immune crosstalk between CD8 + T-cells, which dominates anti-tumor responses, and canine lymphoma has rarely been described. Methods: This study investigates the immune manipulating effects of EVs, produced from the clinical cases and cell line of canine B cell lymphoma, on CD8 + T-cells isolated from canine donors. Results: Lymphoma-derived EVs lead to the apoptosis of CD8 + T-cells. Furthermore, EVs trigger the overexpression of CTLA-4 on CD8 + T-cells, which indicates that EV blockade could serve as a potential therapeutic strategy for lymphoma patients. Notably, EVs transform the CD8 + T-cells into regulatory phenotypes by upregulating their PD-1, PD-L1, and FoxP3 mRNA expression. The regulatory CD8 + T-cells secret the panel of inhibitory cytokines and angiogenic factors and thus create a pro-tumorigenic microenvironment. Conclusion: In summary, the current study demonstrated that the EVs derived from canine B cell lymphoma impaired the anti-tumor activity of CD8 + T-cells and manipulated the possible induction of regulatory CD8 + T-cells to fail the activation of host cellular immunity. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/637262 | ISSN: | 1475-2867 | DOI: | 10.1186/s12935-023-03104-4 |
顯示於: | 獸醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。