Hybridization Protection Reaction for Sensitive and Robust Gene Expression Profiling of Clinical Formalin-Fixed Paraffin-Embedded Samples
Journal
Clinical chemistry
Journal Volume
69
Journal Volume
69
Journal Issue
12
Journal Issue
12
Start Page
1385
End Page
1395
ISSN
00099147
Date Issued
2023-12-01
Author(s)
Abstract
RNA profiling of formalin-fixed paraffin-embedded (FFPE) tumor tissues for the molecular diagnostics of disease prognosis or treatment response is often irreproducible and limited to a handful of biomarkers. This has led to an unmet need for robust multiplexed assays that can profile several RNA biomarkers of interest using a limited amount of specimen. Here, we describe hybridization protection reaction (HPR), which is a novel RNA profiling approach with high reproducibility.
SDGs
Type
journal article