https://scholars.lib.ntu.edu.tw/handle/123456789/637881
標題: | Neuroprotective effects of nobiletin and tangeretin against amyloid β<inf>1-42</inf>-induced toxicity in cultured primary rat neurons | 作者: | Hung, Wei Lun Chiu, Tsao Hsiang Wei, Guor Jien MIN-HSIUNG PAN Ho, Chi Tang Hwang, Lucy Sun Hsu, Bo Yang |
關鍵字: | Alzheimer’s disease | Neuroprotective effects | Nobiletin | Tangeretin | 公開日期: | 1-十二月-2023 | 卷: | 48 | 期: | 2 | 來源出版物: | Nutrire | 摘要: | Background: Amyloid β (Aβ)-induced oxidative stress and neurotoxicity play an important role in the pathogenesis of Alzheimer’s disease (AD). In this study, we evaluated the neuroprotective effects of nobiletin and tangeretin against Aβ-induced neurotoxicity in primary rat neurons. Methods: The protection of nobiletin and tangeretin against Aβ1-42 toxicity on primary cortical neurons was determined by the MTT assay. The free radical scavenging ability of nobiletin and tangeretin was evaluated by measuring the intensity of dichlorodihydrofluorescein (DCF) fluorescence. The protective effects of nobiletin and tangeretin against Aβ1-42-induced neurotoxicity were further evaluated by the western blot and thioflavin T binding assay. Results: Nobiletin (1, 5, 12.5, 25, 50 μM) and tangeretin (1, 5, 12.5, 25 μM) significantly decreased Aβ-induced neurotoxicity in a concentration-dependent manner (P < 0.05 or P < 0.01). The cell viability of neuronal cells decreased to 68.5% by treatment of Aβ1-42 (1 μM). The highest cell viability (81.4%) of nobiletin treatment group was found at the concentration of 50 μM. Meanwhile, tangeretin possessed the highest neuroprotective effect at the concentration of 25 μM in which the cell viability was 88.9%. In addition, nobiletin and tangeretin effectively suppressed Aβ-induced intracellular oxidative stress (P < 0.05). Tangeretin also significantly reduced the aggregation of Aβ1-42 monomer (P < 0.01), while aggregation of Aβ1-42 was not significantly inhibited by treatment with nobiletin. Conclusion: Both nobiletin and tangeretin significantly decreased Aβ1-42-induced neurotoxicity. Tangeretin significantly reduced the aggregation of Aβ1-42, while the neuroprotective effect of nobiletin was due to the reduction of oxidative damage. The results suggested that tangeretin and nobiletin might be a potential neuroprotective food ingredient. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/637881 | ISSN: | 15198928 | DOI: | 10.1186/s41110-023-00241-8 |
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