https://scholars.lib.ntu.edu.tw/handle/123456789/637970
標題: | BCL10GFP fusion protein as a substrate for analysis of determinants required for mucosa-associated lymphoid tissue 1 (MALT1)-mediated cleavage | 作者: | Jou, Shin-Yi Chang, Chien-Chih Wu, Chun-Hsien MEI-RU CHEN CHING-HWA TSAI Chuang, Wen-Hui Chen, Yun-Hui ANN-LII CHENG SHIN-LIAN DOONG |
關鍵字: | BCL10GFP | In vivo | MALT1 | Paracaspase | Processing | 公開日期: | 5-十月-2012 | 卷: | 19 | 期: | 1 | 來源出版物: | Journal of biomedical science | 摘要: | MALT1 belongs to a family of paracaspase and modulates NF-κB signaling pathways through its scaffolding function and proteolytic activity. MALT1 cleaves protein substrates after a positively charged Arginine residue. BCL10, a 233 amino acids polypeptide, is identified as one of the MALT1 proteolytic substrates. MALT1 cleaves BCL10 at the C-terminal end of Arg228. A mere 5 amino acids difference between the substrate and the proteolytic product made it difficult to tell whether the cleavage event took place by using a simple western blot analysis. Here, BCL10GFP was constructed and utilized to examine the specificity and domain determinants for MALT1 cleavage in cells. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/637970 | ISSN: | 10217770 | DOI: | 10.1186/1423-0127-19-85 |
顯示於: | 微生物學科所 |
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